Journal: bioRxiv

Article Title: Intraprocedural endothelial cell seeding of arterial stents via biotin/avidin targeting mitigates in-stent restenosis

doi: 10.1101/2022.05.25.493423

Figure Lengend Snippet: Step 1: Metal samples are exposed to aqueous polyallylamine bisphosphonate (PAB) solution resulting in permanent surface modification via formation of coordination bonds between the metal atoms and bisphosphonate groups of PAB to exhibit primary amine groups available for chemical conjugation reactions. Step 2: Aminated metal substrate is reacted with an amine-reactive biotinylation agent (sulfo-NHS-LC-LC-biotin). Step 3: Biotinylated metal substrate is exposed to avidin to attain a monolayer of avidin on the metal surface. Step 4: Endothelial cells are surface modified with biotin via the reaction of sulfo-NHS-LC-LC-biotin with amine group of proteins on the cell surface. Step 5: Biotinylated endothelial cells are brought in contact with avidin-modified metal samples resulting in augmented cell binding to the metal substrate (Step 6).

Article Snippet: Primary rat aortic endothelial cells (RAEC; Cell Applications, San Diego, CA) were cultured in EGM2 medium (Lonza, Walkersville, MD) and used at passages 4-7.

Techniques: Modification, Conjugation Assay, Avidin-Biotin Assay, Binding Assay